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a-d: Subcellular localization of mV-tagged PLT3, ELF3, PIF3, and PIF4 <t>in</t> <t>HEp-2</t> cells. e-e’’: ELF3-mV (yellow) localizes to cytoplasmic condensates when co-expressed with PLT3-mRb (red), which forms nuclear condensates. f-i’’: ELF3-mV (yellow) and PLT3-mV colocalize with PIF3-Cer (cyan) and PIF4-Cer (cyan) in the nucleus within condensates. j-k’’’: FP-tagged ELF3 (yellow) and PLT3 (red) colocalize in the nucleus with PIF3-Cer and PIF4-Cer. Scale bars represent 5 µm. mV= mVenus; mRb= mRuby2; Cer= Cerulean.
Mammalian Human Epithelial Cells Hep 2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a-d: Subcellular localization of mV-tagged PLT3, ELF3, PIF3, and PIF4 <t>in</t> <t>HEp-2</t> cells. e-e’’: ELF3-mV (yellow) localizes to cytoplasmic condensates when co-expressed with PLT3-mRb (red), which forms nuclear condensates. f-i’’: ELF3-mV (yellow) and PLT3-mV colocalize with PIF3-Cer (cyan) and PIF4-Cer (cyan) in the nucleus within condensates. j-k’’’: FP-tagged ELF3 (yellow) and PLT3 (red) colocalize in the nucleus with PIF3-Cer and PIF4-Cer. Scale bars represent 5 µm. mV= mVenus; mRb= mRuby2; Cer= Cerulean.
Human Hep 2 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a-d: Subcellular localization of mV-tagged PLT3, ELF3, PIF3, and PIF4 <t>in</t> <t>HEp-2</t> cells. e-e’’: ELF3-mV (yellow) localizes to cytoplasmic condensates when co-expressed with PLT3-mRb (red), which forms nuclear condensates. f-i’’: ELF3-mV (yellow) and PLT3-mV colocalize with PIF3-Cer (cyan) and PIF4-Cer (cyan) in the nucleus within condensates. j-k’’’: FP-tagged ELF3 (yellow) and PLT3 (red) colocalize in the nucleus with PIF3-Cer and PIF4-Cer. Scale bars represent 5 µm. mV= mVenus; mRb= mRuby2; Cer= Cerulean.
Human Epidermoid Larynx Carcinoma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a-d: Subcellular localization of mV-tagged PLT3, ELF3, PIF3, and PIF4 <t>in</t> <t>HEp-2</t> cells. e-e’’: ELF3-mV (yellow) localizes to cytoplasmic condensates when co-expressed with PLT3-mRb (red), which forms nuclear condensates. f-i’’: ELF3-mV (yellow) and PLT3-mV colocalize with PIF3-Cer (cyan) and PIF4-Cer (cyan) in the nucleus within condensates. j-k’’’: FP-tagged ELF3 (yellow) and PLT3 (red) colocalize in the nucleus with PIF3-Cer and PIF4-Cer. Scale bars represent 5 µm. mV= mVenus; mRb= mRuby2; Cer= Cerulean.
Human Larynx Carcinoma Hep 2 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human hepatoma cell lines hepg2 2 15  (ATCC)
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ATCC human hepatoma cell lines hepg2 2 15
a-d: Subcellular localization of mV-tagged PLT3, ELF3, PIF3, and PIF4 <t>in</t> <t>HEp-2</t> cells. e-e’’: ELF3-mV (yellow) localizes to cytoplasmic condensates when co-expressed with PLT3-mRb (red), which forms nuclear condensates. f-i’’: ELF3-mV (yellow) and PLT3-mV colocalize with PIF3-Cer (cyan) and PIF4-Cer (cyan) in the nucleus within condensates. j-k’’’: FP-tagged ELF3 (yellow) and PLT3 (red) colocalize in the nucleus with PIF3-Cer and PIF4-Cer. Scale bars represent 5 µm. mV= mVenus; mRb= mRuby2; Cer= Cerulean.
Human Hepatoma Cell Lines Hepg2 2 15, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human hepatocellular carcinoma cell line hepg 2  (ATCC)
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a-d: Subcellular localization of mV-tagged PLT3, ELF3, PIF3, and PIF4 <t>in</t> <t>HEp-2</t> cells. e-e’’: ELF3-mV (yellow) localizes to cytoplasmic condensates when co-expressed with PLT3-mRb (red), which forms nuclear condensates. f-i’’: ELF3-mV (yellow) and PLT3-mV colocalize with PIF3-Cer (cyan) and PIF4-Cer (cyan) in the nucleus within condensates. j-k’’’: FP-tagged ELF3 (yellow) and PLT3 (red) colocalize in the nucleus with PIF3-Cer and PIF4-Cer. Scale bars represent 5 µm. mV= mVenus; mRb= mRuby2; Cer= Cerulean.
Human Hepatocellular Carcinoma Cell Line Hepg 2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a-d: Subcellular localization of mV-tagged PLT3, ELF3, PIF3, and PIF4 in HEp-2 cells. e-e’’: ELF3-mV (yellow) localizes to cytoplasmic condensates when co-expressed with PLT3-mRb (red), which forms nuclear condensates. f-i’’: ELF3-mV (yellow) and PLT3-mV colocalize with PIF3-Cer (cyan) and PIF4-Cer (cyan) in the nucleus within condensates. j-k’’’: FP-tagged ELF3 (yellow) and PLT3 (red) colocalize in the nucleus with PIF3-Cer and PIF4-Cer. Scale bars represent 5 µm. mV= mVenus; mRb= mRuby2; Cer= Cerulean.

Journal: bioRxiv

Article Title: EARLY FLOWERING 3 (ELF3): a novel role in integrating environmental stimuli with root stem cell niche maintenance

doi: 10.64898/2026.01.12.699078

Figure Lengend Snippet: a-d: Subcellular localization of mV-tagged PLT3, ELF3, PIF3, and PIF4 in HEp-2 cells. e-e’’: ELF3-mV (yellow) localizes to cytoplasmic condensates when co-expressed with PLT3-mRb (red), which forms nuclear condensates. f-i’’: ELF3-mV (yellow) and PLT3-mV colocalize with PIF3-Cer (cyan) and PIF4-Cer (cyan) in the nucleus within condensates. j-k’’’: FP-tagged ELF3 (yellow) and PLT3 (red) colocalize in the nucleus with PIF3-Cer and PIF4-Cer. Scale bars represent 5 µm. mV= mVenus; mRb= mRuby2; Cer= Cerulean.

Article Snippet: Therefore, we used mammalian Human epithelial cells HEp-2 (epithelial larynx carcinoma, ATCC-CCL-23) as a non-plant orthogonal system for transient protein expression.

Techniques: